日本積水流感病毒診斷試劑盒

廣州健侖生物科技有限公司

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日本積水流感病毒診斷試劑盒

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日本積水

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【公司名稱】 廣州健侖生物科技有限公司
【市場部】    楊永漢

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【騰訊  】 2042552662
【公司地址】 廣州清華科技園創(chuàng)新基地番禺石樓鎮(zhèn)創(chuàng)啟路63號(hào)二期2幢101-103室

（九）粘片
切下來的切片經(jīng)過鏡檢（根據(jù)情況也可不檢），將符合要求的切片粘在潔凈的載玻片上。粘片時(shí)先在載玻片上滴上一小滴粘片劑（可用蛋青和甘油各50ml，加1g水楊酸混合而成），加幾滴蒸餾水，然后用鑷子小心把切片放在水上，在35-40℃下，用撥針將切片伸直、展平，傾斜載片，使水流去并烘干。
（十）脫蠟、染色、脫水透明和封片
粘片后要經(jīng)過脫蠟、染色、再次脫水透明，然后封片*保存。下面以植物生物學(xué)中觀察組織結(jié)構(gòu)zui常用的番紅-固綠染色法說明脫蠟、染色、脫水透明和封片的過程：
二甲苯 →二甲苯（每次均10~15min.）→1/3純酒精+2/3二甲苯（1~2min.）→1/2純酒精+1/2二甲苯（1~2min.）→2/3純酒精+1/3二甲苯（1~2min.）→純酒精兩次（每次1~2min.）→95%、85%、70%、50%梯度酒精復(fù)水（每級(jí)1~2min.或更長）→用1%番紅配50%酒精染色1小時(shí)→50%、70%、85%、95%梯度酒精脫水（每級(jí)1~2min.或更長）→用0.1%固綠配95%酒精復(fù)染2~5秒→95%酒精脫水1~2分鐘→純酒精兩次（每次1~2min.）→2/3純酒精+1/3二甲苯透明（1~2min.）→1/2純酒精+1/2二甲苯透明（1~2min.）→1/3純酒精+2/3二甲苯透明（1~2min.）→二甲苯 →二甲苯（每次均10~15min.）→加拿大樹膠封片→貼標(biāo)簽鏡檢及保存。
以上是石蠟制片的一般步驟；為了獲得良好的制片效果，需要制片者的大量實(shí)踐并在實(shí)踐中不斷摸索、積累經(jīng)驗(yàn)方可。

(Nine) sticky sheet
Cut the slice after microscopic examination (according to the situation may not be seized), will meet the requirements of the sticky piece of clean glass slides. Slice slides on the slides when slimming tablets (usable egg white and glycerol each 50ml, plus 1g salicylic acid mixture), add a few drops of distilled water, and then carefully with tweezers on the water on the slice, At 35-40 ° C, use a needle to straighten the slice, flatten, tilt the slide, and let the water flow and dry.
(10) Dewaxing, dyeing, dewatering and sealing
After the sticky sheet to go through dewaxing, dyeing, dewatering again transparent, and then keep the seal permanently. The process of observing the structure of plant tissues in plant biology, which is most commonly used for safflower-solid green staining, in dewaxing, dyeing, dewatering and sealing, is described below:
Xylene → xylene (each time 10 ~ 15min.) → 1/3 pure alcohol + 2/3 xylene (1 ~ 2min.) → 1/2 pure alcohol and 1/2 xylene (1 ~ 2min. → 2/3 pure alcohol +1/3 xylene (1 ~ 2min.) → pure alcohol twice (1 ~ 2min each time) → 95%, 85%, 70%, 50% gradient alcohol rehydration 1 ~ 2min. Or longer) → stain with 1% Safranin with 50% alcohol for 1 hour → dehydrate with 50%, 70%, 85%, 95% % Solid green with 95% alcohol counterstain 2 ~ 5 seconds → 95% alcohol dehydration 1 ~ 2 minutes → pure alcohol twice (each 1 ~ 2min.) → 2/3 pure alcohol +1/3 xylene transparent ~ 2min.) → 1/2 pure alcohol +1/2 xylene clear (1 ~ 2min.) → 1/3 pure alcohol + 2/3 xylene clear (1 ~ 2min.) → xylene → xylene Average 10 ~ 15min.) → Canadian gland film → labeling and preservation.
The above is the general steps of paraffin production; in order to obtain a good production effect, the producer needs a lot of practice and in practice continue to explore and gain experience before formulating.